Chromomycin Facts Sheet

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alternate names: Chromomycin A3  , Toyomycin  , Aburamycin

Chromomycin  is a Glycosidic antibiotic from streptomyces griseus used as a fluorescent stain of DNA and as an antineoplastic agent.

Pharmacological action: antibiotics, antineoplastic, fluorescent dyes, nucleic acid synthesis inhibitors.

Chemical name: Olivomycin D, 3D-O-(4-O-acetyl-2,6-dideoxy-3-C-methyl-alpha-L-arabino-hexopyranosyl)-7-methyl-



Chromomycin belongs to the DNA-Interactive Drugs

Chromomycin activity: DNA Minor groove binder.  Chromomycin  has been Demonstrated to bind certain sequences as dimer by NMR [Gao, 1989; Gao, 1990], and quantitative DNase I footprinting [Dabrowiak, 1989]

Chromomycin  binds to G/C sequences, usually at least 3 consecutive G/C as determined by footprinting studies [van Dyke, 1983; Fox, 1985; Cons, 1989] and in vitro binding studies with oligonucleotides [Liu, 1994]

 Dimer binding results in wide, shallow minor groove with major groove compression [Gao, 1989]. One report states that DMS methylation pattern is altered [Fox, 1985], but a conflicting report also exists [Dabrowiak, 1989]

Chromomycin : Originally isolated from Streptomyces griseus

Chromomycin and mithramycin are two DNA binding anticancer antibiotics, acting via inhibition of replication and transcription during macromolecular biosynthesis.  The clinical use of chromomycin is limited because of its immuno suppressive properties and  greater cytotoxic effects

chromomycin A3  interacts with chromatin and exert their deleterious effects upon its structure and hence function, with preference to H1 depleted chromatin.  Histones, particularly their N-terminal tail domains stand in the way of free access of these antibiotics to the chromosomal DNA.  



Chromomycin A3 inhibits influenza a virus multiplication in chick embryo fibroblast cells

Differential interactions of antitumor antibiotics chromomycin A(3) and mithramycin with d(TATGCATA)(2) in presence of Mg(2+).

Structural basis of DNA recognition by anticancer antibiotics, chromomycin A(3), and mithramycin: roles of minor groove width and ligand flexibility

Association of the anticancer antibiotic chromomycin A(3) with the nucleosome: role of core histone tail domains in the binding process


[top] This page was done by a student of Bioinformatics, Open University Jerusalem Israel, in a homage to Fermentek and to Dr Berend.
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